Assessing Diagnostic Accuracy of Serum Holotranscobalamin (Energetic-B12) in Comparability with Different Markers of Vitamin B12 Deficiency

 

• About 15-40% India is Vitamin B12 poor (generally identified by whole Vitamin B12) however, as solely holoTC (lively kind) is taken up by physique cells, thus measuring holoTC is extra reflective of Vitamin B12 standing than the previous. We aimed to evaluate diagnostic accuracy of serum holoTC in comparison with whole Vitamin B12 and whole Homocysteine (HCY) as indicator of serum Vitamin B12 standing. 217 human topics (99 males and 118 females) starting from 17 to 83 years had been divided into Vitamin B12 poor (n = 70), borderline (n = 100) and sufficient teams (n = 47) who had been further assessed for markers of Vitamin B12 deficiency-holoTC, HCY, Imply Corpuscular Quantity (MCV), Folate, heamoglobin and creatinine.

 

• Samples had been analysed utilizing Siemens Advia Centaur Xpi. Complete Vitamin B12 poor group had – 84.3% holoTC poor; 15.7% holoTC ample; 72.9% with elevated HCY; 27.1% with regular HCY; 11.4% with megaloblastic anaemia. Borderline group had – 34% holoTC poor; 28% elevated HCY.

 

• A robust optimistic correlation was discovered between Complete Vitamin B12 and holoTC (r = 0.754, p = <0.001) however robust adverse correlation existed between holoTC and HCY (r = – 0.471, p = <0.001). Concordance between Complete Vit B12 and HCY (Kappa index = 0.51, p < 0.001); between holoTC and HCY (Kappa index = 0.52, p = <0.001) had been statically vital however the latter had a greater sensitivity and specificity.

         DKK-1 (HEK293-expressed), Human | Human DKK-1 ELISA package

• Additionally, statically vital affiliation exists between Complete Vitamin B12 and holoTC with HCY (p = <0.001). Due to this fact, it’s ascertained that Energetic Vitamin B12 assay is a better check and will be thought-about as an early marker of vitamin B12 deficiency.

  Key phrases: Energetic vitamin B12; Holotranscobalamin (holoTC); Homocystine (HCY); Vitamin B12; Vitamin B12 deficiency.

 

 

Dkk-1
GT30000	Neuromics	50 ug	EUR 526

DKK-1 ELISA KIT|Human
EF000090	Lifescience Market	96 Tests	EUR 689

Human DKK-1 ELISA kit
LF-EK50797	Abfrontier	1×96T	EUR 648

DKK-1 (HEK293-expressed), Human
HY-P7155A	MedChemExpress	50ug	EUR 843

Recombinant Human DKK-1 Protein
PROTO94907-3	BosterBio	10ug	EUR 317

Dkk-1 Polyclonal Antibody
ES4292-100ul	ELK Biotech	100ul	EUR 279

Dkk-1 Polyclonal Antibody
ES4292-50ul	ELK Biotech	50ul	EUR 207

Dkk-1 Polyclonal Antibody
ABP53293-003ml	Abbkine	0.03ml	EUR 158

Dkk-1 Polyclonal Antibody
ABP53293-01ml	Abbkine	0.1ml	EUR 289

Dkk-1 Polyclonal Antibody
ABP53293-02ml	Abbkine	0.2ml	EUR 414

Anti-Dkk-1 antibody
STJ97257	St John's Laboratory	200 µl	EUR 197

Anti-Dkk-1 antibody
STJ98000	St John's Laboratory	100 µl	EUR 234

Dkk-1 Polyclonal Antibody
41928-50ul	SAB	50ul	EUR 187

Dkk-1 Polyclonal Antibody
41928-100ul	SAB	100ul	EUR 252

Human CellExp? DKK-1, Human Recombinant
7132-10	Biovision		EUR 278

Human CellExp? DKK-1, Human Recombinant
7132-50	Biovision		EUR 1132

Dkk-2
GT15222	Neuromics	100 ug	EUR 526

Human DKK-1 PicoKine ELISA Kit
EK0867	BosterBio	96 wells	EUR 425

ELISA kit for Human DKK-1
EK5390	SAB	96 tests	EUR 553

DKK-1 (CHO-expressed), Mouse
HY-P7154	MedChemExpress	50ug	EUR 762

Recombinant Mouse Dkk-1 Protein
RP01062	Abclonal	5 μg	EUR 183

Dkk-1 Polyclonal Conjugated Antibody
C41928	SAB	100ul	EUR 397

Human DKK-1 ELISA kit (4×96T)
LF-EK50798	Abfrontier	4×96T	EUR 2201

Dkk-3 antibody
22898-100ul	SAB	100ul	EUR 390

DKK-3 ELISA KIT|Human
EF000091	Lifescience Market	96 Tests	EUR 689

Recombinant Human Dkk-3 Protein
RP00355	Abclonal	10 μg	EUR 164

ELISA kit for Rat DKK-1
EK5668	SAB	96 tests	EUR 553

Human DKK-1(Dickkopf-related protein 1) ELISA Kit
EH0113	FN Test	96T	EUR 524.1

Dkk-1 ELISA Kit (Human) : 96 Wells (OKAG00221)
OKAG00221	Aviva Systems Biology	96 Wells	EUR 596

Dkk-3 Polyclonal Antibody
ES5541-50ul	ELK Biotech	50ul	EUR 207

Dkk-3 Polyclonal Antibody
ES5541-100ul	ELK Biotech	100ul	EUR 279

Dkk-3 Polyclonal Antibody
ABP54542-003ml	Abbkine	0.03ml	EUR 158

Dkk-3 Polyclonal Antibody
ABP54542-01ml	Abbkine	0.1ml	EUR 289

Dkk-3 Polyclonal Antibody
ABP54542-02ml	Abbkine	0.2ml	EUR 414

Anti-Dkk-3 antibody
STJ92720	St John's Laboratory	200 µl	EUR 197

Anti-Dkk-3 antibody
STJ98001	St John's Laboratory	100 µl	EUR 234

Human DKK-4 PicoKine ELISA Kit
EK0868	BosterBio	96 wells	EUR 455

Recombinant Human Dkk-3/DKK3 Protein
RP00209	Abclonal	10 μg	EUR 155

DKK-3 ELISA Kit (Human) (OKBB00603)
OKBB00603	Aviva Systems Biology	96 Wells	EUR 505

Recombinant Dkk-1 Protein (Thr 32-His 266) [Fc]
VAng-1451Lsx-100g	Creative Biolabs	100 µg	EUR 848

Recombinant Dkk-1 Protein (Thr 32-His 266) [Fc]
VAng-1451Lsx-1mg	Creative Biolabs	1 mg	EUR 4724

Recombinant Dkk-1 Protein (Thr 32-His 266) [His]
VAng-1453Lsx-100g	Creative Biolabs	100 µg	EUR 1013

Recombinant Dkk-1 Protein (Thr 32-His 266) [His]
VAng-1453Lsx-1mg	Creative Biolabs	1 mg	EUR 4999

Recombinant Dkk-1 Protein (Thr 32-His 266) [Strep]
VAng-1454Lsx-1mg	Creative Biolabs	1 mg	EUR 6374

Recombinant Dkk-1 Protein (Thr 32-His 266) [Strep]
VAng-1454Lsx-250g	Creative Biolabs	250 µg	EUR 2470

Recombinant Dkk-1 Protein (Thr 32-His 266) [Strep]
VAng-1454Lsx-25g	Creative Biolabs	25 µg	EUR 765

Recombinant Dkk-1 Protein (Thr 32-His 272) [His]
VAng-1455Lsx-500g	Creative Biolabs	500 µg	EUR 4449

Recombinant Dkk-1 Protein (Thr 32-His 272) [His]
VAng-1455Lsx-50g	Creative Biolabs	50 µg	EUR 1013

Recombinant Dkk-1 Protein (Thr 32-His 266) [Fc] [Avi]
VAng-1452Lsx-200g	Creative Biolabs	200 µg	EUR 3844

Recombinant Dkk-1 Protein (Thr 32-His 266) [Fc] [Avi]
VAng-1452Lsx-25g	Creative Biolabs	25 µg	EUR 1013

Human DKK-3(Dickkopf-related protein 3) ELISA Kit
EH0114	FN Test	96T	EUR 567.6

Recombinant Dkk-3 Protein (Pro 23-Ile 349) [His]
VAng-1456Lsx-1mg	Creative Biolabs	1 mg	EUR 5494

Recombinant Dkk-3 Protein (Pro 23-Ile 349) [His]
VAng-1456Lsx-50g	Creative Biolabs	50 µg	EUR 848

Recombinant Dkk-3 Protein (Pro 23-Ile 350) [His]
VAng-1457Lsx-200g	Creative Biolabs	200 µg	EUR 1123

Recombinant Dkk-3 Protein (Pro 23-Ile 350) [His]
VAng-1457Lsx-20g	Creative Biolabs	20 µg	EUR 380

Hemokinin 1 (human)
B5318-1	ApexBio	1 mg	EUR 340

Haptoglobin, (Phenotype 1-1) Human Plasma
7536-1	Biovision		EUR 321

Endothelin-1 (1-15), amide, human
A1111-1	ApexBio	1 mg	EUR 722

Recombinant Human Dickkopf-Related Protein 2/DKK-2 (N-Fc, C-6His)
CC49-10ug	Novoprotein	10ug	EUR 156

Recombinant Human Dickkopf-Related Protein 2/DKK-2 (N-Fc, C-6His)
CC49-1mg	Novoprotein	1mg	EUR 2283

Recombinant Human Dickkopf-Related Protein 2/DKK-2 (N-Fc, C-6His)
CC49-500ug	Novoprotein	500ug	EUR 1613

Recombinant Human Dickkopf-Related Protein 2/DKK-2 (N-Fc, C-6His)
CC49-50ug	Novoprotein	50ug	EUR 369

BNP (1-32), human
A1105-1	ApexBio	1 mg	EUR 177

IGF-1, human recombinant
P1016-.1	ApexBio	100 µg	EUR 763

Neuregulin/Heregulin-1? (NRG-1?/HRG-1?), human recombinant protein
P1054-1	ApexBio	1 mg	EUR 3947

IL1RL1 Human, Interleukin-1 Receptor Like-1 Human Recombinant Protein, Sf9
PROTQ01638-1	BosterBio	Regular: 10ug	EUR 317

MMP-1 Matrix Metalloproteinase-1 Human Recombinant Protein
PROTP03956-1	BosterBio	Regular: 20ug	EUR 317

PSG1 Human, Pregnancy Specific Beta-1-Glycoprotein 1 Human Recombinant Protein, Sf9
PROTP11464-1	BosterBio	Regular: 10ug	EUR 317

ORM1 Orosomucoid 1 Human protein
PROTP02763-1	BosterBio	Regular: 10ug	EUR 317

Recombinant Human Galectin-1 Protein
PROTP09382-1	BosterBio	50ug	EUR 317

Recombinant Human Gremlin-1 Protein
PROTO60565-1	BosterBio	50ug	EUR 317

Parathyroid hormone (1-34) (human)
A1129-1	ApexBio	1 mg	EUR 166

Recombinant Human PECAM-1 Protein
PROTP16284-1	BosterBio	50ug	EUR 317

Recombinant Human ANG-1 Protein
PROTQ15389-1	BosterBio	20ug	EUR 317

Amyloid ?-Peptide (1-42) (human)
B6057-.1	ApexBio	100 ug	EUR 276

ApoA-1, human recombinant protein
P1052-.1	ApexBio	100 µg	EUR 313

ApoA-1, human recombinant protein
P1052-1	ApexBio	1 mg	EUR 1553

WNT-1, human recombinant protein
P1068-1	ApexBio	1 mg	EUR 6940

(1-328) RAD51D (1-328 a.a.) Human Recombinant Protein
PROTO75771-1	BosterBio	Regular: 10ug	EUR 317

NXPH1 Human, Neurexophilin 1 Human Recombinant Protein, Sf9
PROTP58417-1	BosterBio	Regular: 10ug	EUR 317

Falsely optimistic fourth technology ADVIA Centaur® HIV Antigen/Antibody Combo assay within the presence of autoimmune hepatitis sort I (AIH)

 

• A 51-year-old lady was admitted to the hospital with abdominal ache, jaundice, and transaminitis. The affected person’s laboratory outcomes confirmed elevated liver enzymes, excessive antinuclear antibodies (ANA) titer, optimistic anti-smooth muscle antibody, and hypergammaglobulinemia.

 

• Given danger components for HIV an infection, an ADVIA Centaur® HIV Antigen/Antibody Combo assay was carried out exhibiting a reactive pattern with a observe up HIV-1 nucleic acid test (NAT) proving to be adverse.

         Human CellExp? DKK-1, Human Recombinant

• Following affirmation of autoimmune hepatitis sort I through a liver biopsy, steroids had been initiated and vital medical enchancment of signs in addition to decision in transaminitis had been famous.

 

• Autoimmunity is the most certainly causative consider inducing a false optimistic reactive screening assay. You will need to acknowledge that cross-reactivity with autoimmune situations and HIV particular proteins is a possible concern for false reactive samples.

      Recombinant Dkk-1 Protein (Thr 32-His 266) [Fc] [Avi]