Expression and identification of a novel spore wall protein in microsporidian Nosema bombycis
Microsporidia are a group of obligate intracellular parasites causing significant disease in human beings and economically important animals.
Though a few spore wall proteins (SWPs) have now been indentified in these intriguing species, the information on SWPs remains too little to elucidate the spore wall formation mechanisms of microsporidia. It has been well described that numerous proteins with tandem repeats tend to be localized on the cell wall of fungi and parasites.
Recombinant Protein
Previously, by scanning the proteins with tandem repeats in microsporidian Nosema bombycis, we obtained 83 candidate SWPs based on whether those proteins possess a signal peptide and/or transmembrane domain. Here, we further characterized a candidate protein (EOB13250) with three tandem repeats in the N-terminal region and a transmembrane domain in C-terminus of N.
bombycis. Sequence analysis showed that the tandem repeat domain of EOB13250 was species-specific for this parasite. RT-PCR indicated that the expression of the gene encoding this protein started on the fourth day post-infection. After cloned and expressed in E.
Molecular Interaction Between Butorphanol and κ-Opioid Receptor
coli, a polyclone antibody against the recombinant EOB13250 protein was prepared. Western-blotting demonstrated this protein exist in N. bombycis. Immunofluorescence analysis (IFA) and immunoelectron microscopy analysis (IEM) further provided evidence that EOB13250 was an endospore wall protein.
These results together suggested that EOB13250 was a novel spore wall protein of N. bombycis. This study provides a further enrichment of the number of identified spore wall proteins in microsporidia and advances our understanding of the spore wall formation mechanism in these obligate unicellular parasites.
Porcine reproductive and respiratory syndrome virus (PRRSV) is a widely disseminated, macrophage-tropic arterivirus that exhibits profound genetic and pathogenic heterogeneity.
The present study was conducted to determine the complete genome sequences of two novel Korean lineage 1 PRRSV-2 strains, KNU-1901 and KNU-1902, which were isolated from vaccinated pig farms experiencing unusually high morbidity and mortality.
Both isolates contained notable discontinuous 423-nucleotide deletions (DELs) within the genes encoding nonstructural protein 2 (nsp2) and GP3 when compared with the prototype strain VR-2332. In particular, the nsp2 DEL viruses had unique quadripartite discontinuous DEL signatures (111-1-19-9) in nsp2; this is an expanded version of the tripartite 111-1-19 DEL previously identified in virulent lineage 1 PRRSV-2 strains.
Phylogenetic analysis revealed that both novel nsp2 DEL viruses belong to the Korean clade (KOR C) of lineage 1 isolates based on ORF5 but cluster with lineage KOR A strains based on the nsp2 or complete genome sequence. Recombination detection analysis suggested that both novel isolates are recombinants and may have evolved via natural inter-lineage recombination between circulating KOR A and KOR C strains.
) PTPN1 Protein (Recombinant) |
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| 20-abx073070 | Abbexa |
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) PPM1A Protein (Recombinant) |
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| 20-abx073071 | Abbexa |
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) PTP4A3 Protein (Recombinant) |
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| 20-abx073078 | Abbexa |
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) SKP1 Protein (Recombinant) |
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| 20-abx073140 | Abbexa |
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) Ketohexokinase Protein (Recombinant) |
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| 20-abx073142 | Abbexa |
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) NGAL Protein (Recombinant) |
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| 20-abx073150 | Abbexa |
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) DDAH1 Protein (Recombinant) |
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| 20-abx073166 | Abbexa |
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) PTP4A1 Protein (Recombinant) |
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| 20-abx073184 | Abbexa |
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) PPP3R1 Protein (Recombinant) |
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| 20-abx073192 | Abbexa |
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) PPM1F Protein (Recombinant) |
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| 20-abx073211 | Abbexa |
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) PPME1 Protein (Recombinant) |
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| 20-abx073232 | Abbexa |
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) PPP4C Protein (Recombinant) |
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| 20-abx073279 | Abbexa |
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) ACAD8 Protein (Recombinant) |
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| 20-abx073280 | Abbexa |
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) ACAT2 Protein (Recombinant) |
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| 20-abx073281 | Abbexa |
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) RNASE3 Protein (Recombinant) |
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| 20-abx073373 | Abbexa |
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) MAPKAPK3 Protein (Recombinant) |
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| 20-abx073468 | Abbexa |
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) PRKAB2 Protein (Recombinant) |
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| 20-abx073469 | Abbexa |
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) ATF3 Protein (Recombinant) |
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| 20-abx073484 | Abbexa |
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) DYRK1A Protein (Recombinant) |
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| 20-abx073488 | Abbexa |
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) PTPN6 Protein (Recombinant) |
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| 20-abx073509 | Abbexa |
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NGAL Protein (Recombinant) |
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| 20-abx073513 | Abbexa |
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NGAL Protein (Recombinant) |
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| 20-abx073525 | Abbexa |
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) PTPMT1 Protein (Recombinant) |
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| 20-abx073545 | Abbexa |
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) PPP1R14A Protein (Recombinant) |
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| 20-abx073553 | Abbexa |
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) PPP1R2 Protein (Recombinant) |
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| 20-abx073582 | Abbexa |
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) PRKA1RA Protein (Recombinant) |
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| 20-abx073603 | Abbexa |
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) PPARG Protein (Recombinant) |
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| 20-abx073662 | Abbexa |
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) PPP3R2 Protein (Recombinant) |
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| 20-abx073701 | Abbexa |
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) ACADL Protein (Recombinant) |
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| 20-abx073728 | Abbexa |
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) ACADVL Protein (Recombinant) |
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| 20-abx073752 | Abbexa |
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) ACADS Protein (Recombinant) |
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| 20-abx073767 | Abbexa |
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) ACADM Protein (Recombinant) |
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| 20-abx073777 | Abbexa |
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) ACADSB Protein (Recombinant) |
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| 20-abx073808 | Abbexa |
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) PPP1R8 Protein (Recombinant) |
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| 20-abx073809 | Abbexa |
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) ATF4 Protein (Recombinant) |
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| 20-abx073858 | Abbexa |
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) CDK2 Protein (Recombinant) |
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| 20-abx073859 | Abbexa |
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) FGFR1 Protein (Recombinant) |
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| 20-abx073878 | Abbexa |
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) Ribokinase Protein (Recombinant) |
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| 20-abx073896 | Abbexa |
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) ADK Protein (Recombinant) |
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| 20-abx073897 | Abbexa |
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) PTP4A2 Protein (Recombinant) |
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| 20-abx073900 | Abbexa |
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) PTPN7 Protein (Recombinant) |
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| 20-abx073941 | Abbexa |
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) PPP3CA Protein (Recombinant) |
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| 20-abx073950 | Abbexa |
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) PPP1CA Protein (Recombinant) |
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| 20-abx073956 | Abbexa |
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) ABHD14B Protein (Recombinant) |
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| 20-abx073961 | Abbexa |
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) PPP1CC Protein (Recombinant) |
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| 20-abx073968 | Abbexa |
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) PTPRC Protein (Recombinant) |
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| 20-abx073990 | Abbexa |
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 SCCA recombinant protein |
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| E62C01501 | EnoGene | 20ug | EUR 382.00 |
 SCCA1 recombinant protein |
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| E62C01502 | EnoGene | 20ug | EUR 382.00 |
 SCCA2 recombinant protein |
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| E62C01503 | EnoGene | 20ug | EUR 382.00 |
 P53 recombinant protein |
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| E62C02301 | EnoGene | 20ug | EUR 382.00 |
 DKK1 recombinant protein |
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| E62C02401 | EnoGene | 20ug | EUR 382.00 |
 Recombinant Protein G |
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| 7-05935 | CHI Scientific | 1mg | Ask for price |
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Recombinant Protein G |
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| 7-05936 | CHI Scientific | 10mg | Ask for price |
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Recombinant Protein G |
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| 7-05937 | CHI Scientific | 100mg | Ask for price |
 Recombinant Protein A |
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| DAG390 | Creative Diagnostics | 1g | EUR 1940.00 |
 KIAA0513Human Recombinant Protein |
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| PROTO60268 | BosterBio | Regular: 10ug | EUR 317.00 |
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Description: KIAA0513 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 424 amino acids (1-401 a.a) and having a molecular mass of 47.9kDa.;KIAA0513 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
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 Batroxobin Recombinant Protein |
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| PROTP04971 | BosterBio | Regular: 10ug | EUR 317.00 |
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Description: The Batroxobin Recombinant Protein, produced in yeast, is a single, glycosilated polypeptide chain containing 231 amino acids and having an Mw of approximately 28-33 kDa. |
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 Lysostaphin Recombinant Protein |
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| PROTP10547 | BosterBio | Regular: 5mg | EUR 366.00 |
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Description: Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination.;The Mw of lysostaphin is 26,921 (Recsei et al, PNAS 1987). |
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 Streptavidin Recombinant Protein |
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| PROTP22629-2 | BosterBio | Regular: 20mg | EUR 317.00 |
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Description: Streptavidin Streptomyces Avidinii Recombinant produced in E.Coli. ;The molecular weight per tetramer is approximately 52kDa. |
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 Staphylokinase Recombinant Protein |
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| PROTP68802 | BosterBio | Regular: 100ug | EUR 317.00 |
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Description: Staphylokinase Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 136 amino acids and having a molecular weight of 16kDa.;The Staphylokinase is purified by proprietary chromatographic techniques. |
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 TIAL1Human Recombinant Protein |
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| PROTQ01085 | BosterBio | Regular: 20ug | EUR 317.00 |
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Description: TIAL1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 398 amino acids (1-375 a.a) and having a molecular mass of 44.0kDa.TIAL1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
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 LIN7BHuman Recombinant Protein |
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| PROTQ9HAP6 | BosterBio | Regular: 10ug | EUR 317.00 |
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Description: LIN7B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 230 amino acids (1-207 a.a.) and having a molecular mass of 25.3kDa.;LIN7B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
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Interestingly, compared with the prototype VR-2332 virus, the novel nsp2 DEL variants were less efficient at promoting the expression of immune response genes in porcine alveolar macrophage culture. Taken together, we conclude that KNU-1901 and KNU-1902 are recently evolved recombinant variants of the virulent lineage 1 family that caused the regional severe PRRS outbreaks.