Protein Phosphates alpha and beta, Monoclonal Antibody

Immune response of rat spleen cells to a carcinogen and to vaccination with anti-p53 polyclonal antibodies

Beforehand, we reported that human p53 is functionally inactivated by S-glutathionylation at Cys-141 throughout oxidative and DNA-damaging remedies.
Right here, we describe the presence of thiolated p53 and the dynamic nature of this modification in human tissues utilizing distinctive and particular polyclonal antibodies raised towards a 12-residue p53 peptide bearing a blended disulfide at Cys-141.
The affinity- purified antibodies (glut-p53) have been sequence-specific in that they acknowledged the antigenic peptide however not the unthiolated peptide or a scrambled glutathionylated peptide in ELISAs.
On immunoblots, the purified antibodies didn’t react with native p53 or recombinant p53 (rp53), however readily detected the glutathionylated or cysteinylated or ethanethiol-treated rp53 solely below nonreducing circumstances.
Untreated HCT116 cells confirmed low ranges of glut-p53, which elevated markedly after H(2)O(2), diamide, cisplatin, and doxorubicin remedies.
Glut-p53 ranges decreased sharply Protein Phosphates after cells have been handed into oxidant-free medium, suggesting environment friendly dethiolation. The mutant p53 current in HT29 and T47D human most cancers cells was additionally acknowledged. In vitro, the glut-p53 was quickly degraded by rabbit reticulocyte lysates.
Human prostate and prostate most cancers tissues confirmed an considerable presence of glut-p53 in luminal epithelium, a web site well-known to generate ROS. Melanoma and colon most cancers samples have been additionally optimistic for glut-p53.
The supply of the thiolation-specific antibodies ought to improve our information of p53 regulation in redox-perturbed states present in varied illnesses together with most cancers.
Though the tumor suppressor protein p53 is vital within the management of varied mobile actions, the evaluation of p53 within the porcine mannequin has been hampered by an absence of an appropriate antibody that’s particular for porcine p53.
Utilizing a recombinant porcine p53, we Protein Phosphates generated a rabbit polyclonal antibody (designated SH0797) that’s directed towards porcine p53. The outcomes of the research present that the antibody is able to detecting recombinant p53 protein expressed in Escherichia coli, in addition to FLAG-tagged p53 that’s expressed within the transfected cells.
This demonstrates that the antibody is particular for the porcine p53 protein. The antibody additionally confirmed the flexibility to immunoprecipitate p53 protein from extracts of porcine cells and to cross-react with human p53 protein.
As well as, expression of porcine p53 could possibly be induced readily in porcine cells and detected utilizing this new instrument. This antibody is a great tool to be used in research of the mobile pathways that contain p53 within the porcine mannequin.

Polyclonal Antibody

The cDNA encoding the wild sort p53 protein from flounder, Platichthys flesus, was expressed in Escherichia coli utilizing the GST fusion protein system. A number of milligrams of recombinant p53 protein have been purified.
This protein displayed an obvious molecular weight of 45,000 Da, a price which is similar to Xenopus p53, however considerably larger than was anticipated primarily based on the size of the open studying body.
Immunization of rabbits Protein Phosphates towards purified p53 protein allowed the manufacturing of excessive titre polyclonal antiserum. This new polyclonal antibody acknowledged recombinant flounder p53 protein in Western blot.
PP1
1927-5
EUR 272.00
PP1
20-abx076789
  • EUR 899.00
  • EUR 356.00
  • 25 mg
  • 5 mg
  • Shipped within 5-12 working days.
PP1
HY-13804 10mg
EUR 215.00
PP1
GK9287-1MG 1 mg
EUR 114.00
PP1
GK9287-5MG 5 mg
EUR 252.00
Phospho- PP1
ABD2990 100 ug
EUR 438.00
3MB-PP1
C3340-10 10 mg
EUR 292.00
Description: 3MB-PP1 is a cell-permeable, potent, ATP-competitive, and highly selective inhibitor of polo-like kinase. Human polo-like kinase 1 (PLK1) plays dominant role in mitosis and the maintenance of genomic stability.
3MB-PP1
C3340-5 5 mg
EUR 180.00
Description: 3MB-PP1 is a cell-permeable, potent, ATP-competitive, and highly selective inhibitor of polo-like kinase. Human polo-like kinase 1 (PLK1) plays dominant role in mitosis and the maintenance of genomic stability.
Human Agouti-Related Peptide (AgRP) (71-132), Form C amide ELISA Kit
DEIA-PP1 96T
EUR 1053.00
Description: This kit is designed to measure the concentration of a specific peptide and its related peptides based on the principle of a "competitive" enzyme immunoassay.
1-Naphthyl PP1
A8605-10 10 mg
EUR 254.00
Description: Selective inhibitor of src family kinases v-Src and c-Fyn as well as the tyrosine kinase c-Abl. (IC50 values are 1.0, 0.6, 0.6, 18 and 22 ?M for v-Src, c-Fyn, c-Abl, CDK2 and CAMK II respectively). Preferentially inhibits mutant over wild-type kinases (IC
1-Naphthyl PP1
A8605-5 5 mg
EUR 166.00
Description: Selective inhibitor of src family kinases v-Src and c-Fyn as well as the tyrosine kinase c-Abl. (IC50 values are 1.0, 0.6, 0.6, 18 and 22 ?M for v-Src, c-Fyn, c-Abl, CDK2 and CAMK II respectively). Preferentially inhibits mutant over wild-type kinases (IC
1-Naphthyl PP1
A8605-50 50 mg
EUR 870.00
Description: Selective inhibitor of src family kinases v-Src and c-Fyn as well as the tyrosine kinase c-Abl. (IC50 values are 1.0, 0.6, 0.6, 18 and 22 ?M for v-Src, c-Fyn, c-Abl, CDK2 and CAMK II respectively). Preferentially inhibits mutant over wild-type kinases (IC
PP1 gamma antibody
23054-100ul 100ul
EUR 390.00
1-NM-PP1
B2346-1
EUR 142.00
1-NM-PP1
B2346-5
EUR 414.00
1-NM-PP1
B1299-10 10 mg
EUR 215.00
Description: 1-NM-PP1 is a C3-modified analog of cell-permeable PP1, acts as a potent and selective inhibitor of protein kinase families [1].1-NM-PP1 has shown the selective inhibition of protein kinases that have been mutated.
1-NM-PP1
B1299-25 25 mg
EUR 435.00
Description: 1-NM-PP1 is a C3-modified analog of cell-permeable PP1, acts as a potent and selective inhibitor of protein kinase families [1].1-NM-PP1 has shown the selective inhibition of protein kinases that have been mutated.
1-NM-PP1
B1299-5 5 mg
EUR 154.00
Description: 1-NM-PP1 is a C3-modified analog of cell-permeable PP1, acts as a potent and selective inhibitor of protein kinase families [1].1-NM-PP1 has shown the selective inhibition of protein kinases that have been mutated.
1-NM-PP1
B1299-5.1 10 mM (in 1mL DMSO)
EUR 171.00
Description: 1-NM-PP1 is a C3-modified analog of cell-permeable PP1, acts as a potent and selective inhibitor of protein kinase families [1].1-NM-PP1 has shown the selective inhibition of protein kinases that have been mutated.
1-NM-PP1
B1299-50 50 mg
EUR 689.00
Description: 1-NM-PP1 is a C3-modified analog of cell-permeable PP1, acts as a potent and selective inhibitor of protein kinase families [1].1-NM-PP1 has shown the selective inhibition of protein kinases that have been mutated.
PP1 beta Antibody
20-abx141585
  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.
PP1 alpha Antibody
20-abx217889
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
1-Naphthyl PP1
HY-13941 10mM/1mL
EUR 163.00
1-NM-PP1
HY-13942 10mM/1mL
EUR 194.00
PP1 alpha antibody
70R-31532 100 ug
EUR 327.00
Description: Rabbit polyclonal PP1 alpha antibody
PP1? Polyclonal Antibody
ES6690-100ul 100ul
EUR 279.00
Description: A Rabbit Polyclonal antibody against PP1? from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
PP1? Polyclonal Antibody
ES6690-50ul 50ul
EUR 207.00
Description: A Rabbit Polyclonal antibody against PP1? from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
PP1 alpha Antibody
DF2991 200ul
EUR 304.00
Description: PP1α Antibody detects endogenous levels of total PP1α.
1-Naphthyl PP1 (hydrochloride)
HY-13941B 100mg
EUR 1290.00
PP1 alpha antibody (Thr320)
70R-31531 100 ug
EUR 327.00
Description: Rabbit polyclonal PP1 alpha antibody (Thr320)
PP1 alpha Blocking Peptide
DF2991-BP 1mg
EUR 195.00
PP1 (Src kinase inhibitor)
SIH-469-25MG 25 mg
EUR 619.00
  • PP1 is a potent and selective Src family protein tyrosine kinase inhibitor. This has a range of effects and implications. Structural studies have revealed that PP1 binds to the ATP-binding site in tyrosine kinases and Ser/Thr kinases. PP1 displays >
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Description: The substance PP1 is a src kinase inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is tan solid which is soluble in DMSO (25 mg/ml), slightly soluble in 100% ethanol.
PP1 (Src kinase inhibitor)
SIH-469-5MG 5 mg
EUR 205.00
  • PP1 is a potent and selective Src family protein tyrosine kinase inhibitor. This has a range of effects and implications. Structural studies have revealed that PP1 binds to the ATP-binding site in tyrosine kinases and Ser/Thr kinases. PP1 displays >
  • Show more
Description: The substance PP1 is a src kinase inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is tan solid which is soluble in DMSO (25 mg/ml), slightly soluble in 100% ethanol.
Phospho-PP1 alpha (Thr320) Antibody
20-abx217749
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Anti-PP1 alpha Monoclonal Antibody
A02801 100ul
EUR 397.00
Description: Mouse Monoclonal Antibody for PP1 alpha Antibody (PPP1CA) detection.tested for IF, IHC, WB in Human, Mouse, Rat, Bovine, Chicken, Pig, Rabbit, Zebrafish.
Anti-PP1 beta Monoclonal Antibody
A04022-1 100ul
EUR 397.00
Description: Mouse Monoclonal Antibody for PP1 beta Antibody (PPP1CB) detection. Tested with WB in Human, Mouse, Rat, Chicken, Dog, Pig.
PP1? (phospho Thr320) Polyclonal Antibody
ES6689-100ul 100ul
EUR 279.00
Description: A Rabbit Polyclonal antibody against PP1? (phospho Thr320) from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA
PP1? (phospho Thr320) Polyclonal Antibody
ES6689-50ul 50ul
EUR 207.00
Description: A Rabbit Polyclonal antibody against PP1? (phospho Thr320) from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA
Phospho-PP1 alpha (Thr320) Antibody
DF2990 200ul
EUR 304.00
Description: Phospho-PP1α (Thr320) Antibody detects endogenous levels of PP1α only when phosphorylated at Thr320.
Monoclonal PP1 alpha Antibody, Clone: EP1511Y
AMR09423G 0.1ml
EUR 528.00
Description: A Monoclonal antibody against Human PP1 alpha. The antibodies are raised in Rabbit and are from clone EP1511Y. This antibody is applicable in WB and IHC
Monoclonal PP1 beta Antibody, Clone: EP1804Y
AMR09425G 0.1ml
EUR 528.00
Description: A Monoclonal antibody against Human PP1 beta. The antibodies are raised in Rabbit and are from clone EP1804Y. This antibody is applicable in WB and IHC
Kinase-Enhanced PP1 Inhibitor (KEPI) Antibody
20-abx133282
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Phospho-PP1 alpha (Thr320) Blocking Peptide
DF2990-BP 1mg
EUR 195.00
Okadaic acid (PP1 and PP2A inhibitor)
SIH-521-100UG 100 µg
EUR 333.00
  • Okadaic acid is a potent and selective inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Okadaic acid is a naturally occurring polyether toxin that was originally derived from marine dinoflagellates, Prorocentrium spp.. It i
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Description: The substance Okadaic acid is a pp1 and pp2a inhibitor. It is synthetically produced and has a purity of ?98%. The pure substance is white crystalline solid which is soluble in chloroform, ethanol, methanol, acetone or ethyl acetate.
Okadaic acid (PP1 and PP2A inhibitor)
SIH-521-20UG 20 µg
EUR 137.00
  • Okadaic acid is a potent and selective inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Okadaic acid is a naturally occurring polyether toxin that was originally derived from marine dinoflagellates, Prorocentrium spp.. It i
  • Show more
Description: The substance Okadaic acid is a pp1 and pp2a inhibitor. It is synthetically produced and has a purity of ?98%. The pure substance is white crystalline solid which is soluble in chloroform, ethanol, methanol, acetone or ethyl acetate.
Monoclonal PP1 alpha Antibody Phospho (pT320), Clone: EP1512Y
AMR09424G 0.1ml
EUR 528.00
Description: A Monoclonal antibody against Human PP1 alpha Phospho (pT320). The antibodies are raised in Rabbit and are from clone EP1512Y. This antibody is applicable in WB and IHC
Porcine Serine/threonine- protein phosphatase PP1- beta catalyti
ELI-16517p 96 Tests
EUR 928.00
Rabbit Serine/threonine- protein phosphatase PP1- alpha catalyti
ELI-43549Ra 96 Tests
EUR 928.00
Rabbit Serine/threonine- protein phosphatase PP1- beta catalytic
ELI-45374Ra 96 Tests
EUR 928.00
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody
20-abx001011
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.
Serine/threonine-Protein Phosphatase PP1-Alpha Catalytic Subunit (PPP1CA) Antibody
20-abx001796
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody
abx027661-400ul 400 ul
EUR 523.00
  • Shipped within 5-10 working days.
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody
abx027661-80l 80 µl
EUR 286.00
  • Shipped within 5-10 working days.
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody
abx036134-100ug 100 ug
EUR 391.00
  • Shipped within 5-10 working days.
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody
abx036135-100ug 100 ug
EUR 391.00
  • Shipped within 5-10 working days.
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody
abx037963-100ug 100 ug
EUR 391.00
  • Shipped within 5-10 working days.
Serine/threonine-Protein Phosphatase PP1-Alpha Catalytic Subunit (PPP1CA) Antibody
abx011880-100ul 100 ul
EUR 411.00
  • Shipped within 5-10 working days.
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody
20-abx319355
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Serine/threonine-Protein Phosphatase PP1-Alpha Catalytic Subunit (PPP1CA) Antibody
abx117243-100ug 100 ug
EUR 467.00
  • Shipped within 5-10 working days.
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody
20-abx114823
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.
Serine/threonine-Protein Phosphatase PP1-Alpha Catalytic Subunit (PPP1CA) Antibody
20-abx302367
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody
20-abx302550
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Cross response was additionally noticed with recombinant Xenopus p53 protein however not with human p53 protein. Immunoblotting of the entire protein extract from regular flounder ovaries didn’t reveal any p53 expression.

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