Immune response of rat spleen cells to a carcinogen and to vaccination with anti-p53 polyclonal antibodies
Beforehand, we reported that human p53 is functionally inactivated by S-glutathionylation at Cys-141 throughout oxidative and DNA-damaging remedies.
Right here, we describe the presence of thiolated p53 and the dynamic nature of this modification in human tissues utilizing distinctive and particular polyclonal antibodies raised towards a 12-residue p53 peptide bearing a blended disulfide at Cys-141.
The affinity- purified antibodies (glut-p53) have been sequence-specific in that they acknowledged the antigenic peptide however not the unthiolated peptide or a scrambled glutathionylated peptide in ELISAs.
On immunoblots, the purified antibodies didn’t react with native p53 or recombinant p53 (rp53), however readily detected the glutathionylated or cysteinylated or ethanethiol-treated rp53 solely below nonreducing circumstances.
Untreated HCT116 cells confirmed low ranges of glut-p53, which elevated markedly after H(2)O(2), diamide, cisplatin, and doxorubicin remedies.
Glut-p53 ranges decreased sharply Protein Phosphates after cells have been handed into oxidant-free medium, suggesting environment friendly dethiolation. The mutant p53 current in HT29 and T47D human most cancers cells was additionally acknowledged. In vitro, the glut-p53 was quickly degraded by rabbit reticulocyte lysates.
Human prostate and prostate most cancers tissues confirmed an considerable presence of glut-p53 in luminal epithelium, a web site well-known to generate ROS. Melanoma and colon most cancers samples have been additionally optimistic for glut-p53.
The supply of the thiolation-specific antibodies ought to improve our information of p53 regulation in redox-perturbed states present in varied illnesses together with most cancers.
Polyclonal antibody to porcine p53 protein: a brand new instrument for learning the p53 pathway in a porcine mannequin
Though the tumor suppressor protein p53 is vital within the management of varied mobile actions, the evaluation of p53 within the porcine mannequin has been hampered by an absence of an appropriate antibody that’s particular for porcine p53.
Utilizing a recombinant porcine p53, we Protein Phosphates generated a rabbit polyclonal antibody (designated SH0797) that’s directed towards porcine p53. The outcomes of the research present that the antibody is able to detecting recombinant p53 protein expressed in Escherichia coli, in addition to FLAG-tagged p53 that’s expressed within the transfected cells.
This demonstrates that the antibody is particular for the porcine p53 protein. The antibody additionally confirmed the flexibility to immunoprecipitate p53 protein from extracts of porcine cells and to cross-react with human p53 protein.
As well as, expression of porcine p53 could possibly be induced readily in porcine cells and detected utilizing this new instrument. This antibody is a great tool to be used in research of the mobile pathways that contain p53 within the porcine mannequin.
Polyclonal Antibody
 PP1 |
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| GK9287-1MG | Glentham Life Sciences | 1 mg | EUR 114 |
PP1 |
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| GK9287-5MG | Glentham Life Sciences | 5 mg | EUR 252 |
PP1 |
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| HY-13804 | MedChemExpress | 10mg | EUR 215 |
 Phospho- PP1 |
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| ABD2990 | Lifescience Market | 100 ug | EUR 438 |
 3MB-PP1 |
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| C3340-10 | ApexBio | 10 mg | EUR 292 |
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Description: 3MB-PP1 is a cell-permeable, potent, ATP-competitive, and highly selective inhibitor of polo-like kinase. Human polo-like kinase 1 (PLK1) plays dominant role in mitosis and the maintenance of genomic stability. |
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3MB-PP1 |
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| C3340-5 | ApexBio | 5 mg | EUR 180 |
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Description: 3MB-PP1 is a cell-permeable, potent, ATP-competitive, and highly selective inhibitor of polo-like kinase. Human polo-like kinase 1 (PLK1) plays dominant role in mitosis and the maintenance of genomic stability. |
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 (71-132), Form C amide ELISA Kit) Human Agouti-Related Peptide (AgRP) (71-132), Form C amide ELISA Kit |
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| DEIA-PP1 | Creative Diagnostics | 96T | EUR 1053 |
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Description: This kit is designed to measure the concentration of a specific peptide and its related peptides based on the principle of a "competitive" enzyme immunoassay. |
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 PP1 alpha antibody |
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| 70R-31532 | Fitzgerald | 100 ug | EUR 327 |
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Description: Rabbit polyclonal PP1 alpha antibody |
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 1-NM-PP1 |
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| B2346-1 | Biovision | EUR 142 | |
1-NM-PP1 |
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| B2346-5 | Biovision | EUR 414 | |
 PP1 alpha Antibody |
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| DF2991 | Affbiotech | 200ul | EUR 304 |
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Description: PP1α Antibody detects endogenous levels of total PP1α. |
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 PP1 alpha Antibody |
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| 20-abx217889 | Abbexa |
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 PP1 beta Antibody |
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| 20-abx141585 | Abbexa |
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 PP1 gamma antibody |
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| 23054-100ul | SAB | 100ul | EUR 390 |
 PP1? Polyclonal Antibody |
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| ES6690-100ul | ELK Biotech | 100ul | EUR 279 |
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Description: A Rabbit Polyclonal antibody against PP1? from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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PP1? Polyclonal Antibody |
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| ES6690-50ul | ELK Biotech | 50ul | EUR 207 |
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Description: A Rabbit Polyclonal antibody against PP1? from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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 1-Naphthyl PP1 |
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| HY-13941 | MedChemExpress | 10mM/1mL | EUR 163 |
1-NM-PP1 |
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| HY-13942 | MedChemExpress | 10mM/1mL | EUR 194 |
1-Naphthyl PP1 |
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| A8605-10 | ApexBio | 10 mg | EUR 254 |
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Description: Selective inhibitor of src family kinases v-Src and c-Fyn as well as the tyrosine kinase c-Abl. (IC50 values are 1.0, 0.6, 0.6, 18 and 22 ?M for v-Src, c-Fyn, c-Abl, CDK2 and CAMK II respectively). Preferentially inhibits mutant over wild-type kinases (IC |
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1-Naphthyl PP1 |
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| A8605-5 | ApexBio | 5 mg | EUR 166 |
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Description: Selective inhibitor of src family kinases v-Src and c-Fyn as well as the tyrosine kinase c-Abl. (IC50 values are 1.0, 0.6, 0.6, 18 and 22 ?M for v-Src, c-Fyn, c-Abl, CDK2 and CAMK II respectively). Preferentially inhibits mutant over wild-type kinases (IC |
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1-Naphthyl PP1 |
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| A8605-50 | ApexBio | 50 mg | EUR 870 |
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Description: Selective inhibitor of src family kinases v-Src and c-Fyn as well as the tyrosine kinase c-Abl. (IC50 values are 1.0, 0.6, 0.6, 18 and 22 ?M for v-Src, c-Fyn, c-Abl, CDK2 and CAMK II respectively). Preferentially inhibits mutant over wild-type kinases (IC |
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1-NM-PP1 |
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| B1299-10 | ApexBio | 10 mg | EUR 215 |
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Description: 1-NM-PP1 is a C3-modified analog of cell-permeable PP1, acts as a potent and selective inhibitor of protein kinase families [1].1-NM-PP1 has shown the selective inhibition of protein kinases that have been mutated. |
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1-NM-PP1 |
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| B1299-25 | ApexBio | 25 mg | EUR 435 |
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Description: 1-NM-PP1 is a C3-modified analog of cell-permeable PP1, acts as a potent and selective inhibitor of protein kinase families [1].1-NM-PP1 has shown the selective inhibition of protein kinases that have been mutated. |
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1-NM-PP1 |
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| B1299-5 | ApexBio | 5 mg | EUR 154 |
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Description: 1-NM-PP1 is a C3-modified analog of cell-permeable PP1, acts as a potent and selective inhibitor of protein kinase families [1].1-NM-PP1 has shown the selective inhibition of protein kinases that have been mutated. |
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1-NM-PP1 |
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| B1299-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 171 |
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Description: 1-NM-PP1 is a C3-modified analog of cell-permeable PP1, acts as a potent and selective inhibitor of protein kinase families [1].1-NM-PP1 has shown the selective inhibition of protein kinases that have been mutated. |
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1-NM-PP1 |
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| B1299-50 | ApexBio | 50 mg | EUR 689 |
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Description: 1-NM-PP1 is a C3-modified analog of cell-permeable PP1, acts as a potent and selective inhibitor of protein kinase families [1].1-NM-PP1 has shown the selective inhibition of protein kinases that have been mutated. |
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) PP1 alpha antibody (Thr320) |
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| 70R-31531 | Fitzgerald | 100 ug | EUR 327 |
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Description: Rabbit polyclonal PP1 alpha antibody (Thr320) |
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 PP1 alpha Blocking Peptide |
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| DF2991-BP | Affbiotech | 1mg | EUR 195 |
) PP1 (Src kinase inhibitor) |
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| SIH-469-25MG | Stressmarq | 25 mg | EUR 619 |
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Description: The substance PP1 is a src kinase inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is tan solid which is soluble in DMSO (25 mg/ml), slightly soluble in 100% ethanol. |
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PP1 (Src kinase inhibitor) |
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| SIH-469-5MG | Stressmarq | 5 mg | EUR 205 |
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Description: The substance PP1 is a src kinase inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is tan solid which is soluble in DMSO (25 mg/ml), slightly soluble in 100% ethanol. |
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) 1-Naphthyl PP1 (hydrochloride) |
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| HY-13941B | MedChemExpress | 100mg | EUR 1290 |
 Antibody) Phospho-PP1 alpha (Thr320) Antibody |
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| DF2990 | Affbiotech | 200ul | EUR 304 |
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Description: Phospho-PP1α (Thr320) Antibody detects endogenous levels of PP1α only when phosphorylated at Thr320. |
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 Anti-PP1 beta Monoclonal Antibody |
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| A04022-1 | BosterBio | 100ul | EUR 397 |
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Description: Mouse Monoclonal Antibody for PP1 beta Antibody (PPP1CB) detection. Tested with WB in Human, Mouse, Rat, Chicken, Dog, Pig. |
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 Antibody) Phospho-PP1 alpha (Thr320) Antibody |
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| 20-abx217749 | Abbexa |
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 Anti-PP1 alpha Monoclonal Antibody |
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| A02801 | BosterBio | 100ul | EUR 397 |
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Description: Mouse Monoclonal Antibody for PP1 alpha Antibody (PPP1CA) detection.tested for IF, IHC, WB in Human, Mouse, Rat, Bovine, Chicken, Pig, Rabbit, Zebrafish. |
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 Polyclonal Antibody) PP1? (phospho Thr320) Polyclonal Antibody |
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| ES6689-100ul | ELK Biotech | 100ul | EUR 279 |
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Description: A Rabbit Polyclonal antibody against PP1? (phospho Thr320) from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA |
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PP1? (phospho Thr320) Polyclonal Antibody |
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| ES6689-50ul | ELK Biotech | 50ul | EUR 207 |
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Description: A Rabbit Polyclonal antibody against PP1? (phospho Thr320) from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA |
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 Blocking Peptide) Phospho-PP1 alpha (Thr320) Blocking Peptide |
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| DF2990-BP | Affbiotech | 1mg | EUR 195 |
 Antibody) Kinase-Enhanced PP1 Inhibitor (KEPI) Antibody |
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| 20-abx133282 | Abbexa |
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 Monoclonal PP1 alpha Antibody, Clone: EP1511Y |
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| AMR09423G | Leading Biology | 0.1ml | EUR 528 |
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Description: A Monoclonal antibody against Human PP1 alpha. The antibodies are raised in Rabbit and are from clone EP1511Y. This antibody is applicable in WB and IHC |
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 Monoclonal PP1 beta Antibody, Clone: EP1804Y |
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| AMR09425G | Leading Biology | 0.1ml | EUR 528 |
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Description: A Monoclonal antibody against Human PP1 beta. The antibodies are raised in Rabbit and are from clone EP1804Y. This antibody is applicable in WB and IHC |
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) Okadaic acid (PP1 and PP2A inhibitor) |
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| SIH-521-100UG | Stressmarq | 100 µg | EUR 333 |
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Description: The substance Okadaic acid is a pp1 and pp2a inhibitor. It is synthetically produced and has a purity of ?98%. The pure substance is white crystalline solid which is soluble in chloroform, ethanol, methanol, acetone or ethyl acetate. |
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Okadaic acid (PP1 and PP2A inhibitor) |
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| SIH-521-20UG | Stressmarq | 20 µg | EUR 137 |
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Description: The substance Okadaic acid is a pp1 and pp2a inhibitor. It is synthetically produced and has a purity of ?98%. The pure substance is white crystalline solid which is soluble in chloroform, ethanol, methanol, acetone or ethyl acetate. |
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 Porcine Serine/threonine- protein phosphatase PP1- beta catalyti |
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| ELI-16517p | Lifescience Market | 96 Tests | EUR 928 |
 Rabbit Serine/threonine- protein phosphatase PP1- alpha catalyti |
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| ELI-43549Ra | Lifescience Market | 96 Tests | EUR 928 |
 Rabbit Serine/threonine- protein phosphatase PP1- beta catalytic |
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| ELI-45374Ra | Lifescience Market | 96 Tests | EUR 928 |
, Clone: EP1512Y) Monoclonal PP1 alpha Antibody Phospho (pT320), Clone: EP1512Y |
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| AMR09424G | Leading Biology | 0.1ml | EUR 528 |
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Description: A Monoclonal antibody against Human PP1 alpha Phospho (pT320). The antibodies are raised in Rabbit and are from clone EP1512Y. This antibody is applicable in WB and IHC |
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) Dog Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) |
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| 1-CSB-EP840958DO | Cusabio |
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Description: Recombinant Dog Serine/threonine-protein phosphatase PP1-alpha catalytic subunit(PPP1CA) expressed in E.coli |
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) Human Serine/threonine-protein phosphatase PP1-beta catalytic subunit (PPP1CB) |
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| 1-CSB-EP018504HU | Cusabio |
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Description: Recombinant Human Serine/threonine-protein phosphatase PP1-beta catalytic subunit(PPP1CB) expressed in E.coli |
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 Antibody) Serine/threonine-Protein Phosphatase PP1-Alpha Catalytic Subunit (PPP1CA) Antibody |
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| abx117243-100ug | Abbexa | 100 ug | EUR 467 |
 Antibody) Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody |
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| 20-abx114823 | Abbexa |
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Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody |
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| 20-abx319355 | Abbexa |
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Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody |
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| abx236694-100ug | Abbexa | 100 ug | EUR 481 |
Serine/threonine-Protein Phosphatase PP1-Alpha Catalytic Subunit (PPP1CA) Antibody |
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| 20-abx302367 | Abbexa |
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Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody |
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| 20-abx302550 | Abbexa |
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Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody |
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| abx217902-100ug | Abbexa | 100 ug | EUR 439 |
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody |
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| abx224082-100ug | Abbexa | 100 ug | EUR 411 |
Serine/threonine-Protein Phosphatase PP1-Alpha Catalytic Subunit (PPP1CA) Antibody |
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| 20-abx001796 | Abbexa |
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Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody |
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| abx036134-100ug | Abbexa | 100 ug | EUR 391 |
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody |
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| abx036135-100ug | Abbexa | 100 ug | EUR 391 |
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody |
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| abx037963-100ug | Abbexa | 100 ug | EUR 391 |
Serine/threonine-Protein Phosphatase PP1-Beta Catalytic Subunit (PPP1CB) Antibody |
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| 20-abx001011 | Abbexa |
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